DIFFERENTIATION OF INFECTION FROM VACCINATION IN FOOT-AND-MOUTH-DISEASE BY THE DETECTION OF ANTIBODIES TO THE NONSTRUCTURAL PROTEINS 3D, 3AB AND 3ABC IN ELISA USING ANTIGENS EXPRESSED IN BACULOVIRUS
Kj. Sorensen et al., DIFFERENTIATION OF INFECTION FROM VACCINATION IN FOOT-AND-MOUTH-DISEASE BY THE DETECTION OF ANTIBODIES TO THE NONSTRUCTURAL PROTEINS 3D, 3AB AND 3ABC IN ELISA USING ANTIGENS EXPRESSED IN BACULOVIRUS, Archives of virology, 143(8), 1998, pp. 1461-1476
The baculovirus expression system was found to be efficient at express
ing the 3D, the 3AB and the 3ABC non-structural proteins (NSP) of foot
-and-mouth disease virus (FMDV) as antigens recognised by immune sera
in ELISA. ELISA's using 3D, 3AB and 3ABC detected antibodies from day
8 and 10 after experimental infection of susceptible cattle and sheep
and cattle remained seropositive for more than 395 days. The ELISA's d
etected antibodies against any of the seven serotypes of FMDV. The 3D
ELISA was specific and precise and as sensitive as established ELISA's
which measure antibody to structural proteins. The assay may be used
as a resource saving alternative to established ELISA's for the detect
ion of antibodies against any of the seven serotypes. The 3AB and the
3ABC ELISA were also specific and precise. FMDV infected cattle could
be differentiated from those that had been merely vaccinated as they g
ave a positive result in both the 3AB and the 3ABC ELISA's. Two cattle
that had been both vaccinated and infected also gave, positive result
s in both tests, suggesting that the 3AB and 3ABC ELISA's, but not the
3D ELISA might represent a reliable means of detecting infection in a
vaccinated population.