Y. Aikawa et al., A NEW ANTIRHEUMATIC DRUG, T-614, EFFECTIVELY SUPPRESSES THE DEVELOPMENT OF AUTOIMMUNE ENCEPHALOMYELITIS, Journal of neuroimmunology, 89(1-2), 1998, pp. 35-42
In the present study, we examined the therapeutic effects of T-614 hyl
sulfonylamino-6-phenoxy-4H-1-benzopyran-4-one), a new anti-rheumatic d
rug, on a T cell-mediated autoimmune disease, experimental autoimmune
encephalomyelitis (EAE). T-614 dose-dependently suppressed the develop
ment of active EAE induced in Lewis rats by immunization with myelin b
asic protein (MBP) when administered for 2 weeks starting on the day o
f immunization (day 0 to 14). Amelioration of clinical signs was also
obtained by the treatment at the effector phase (day 7 to 14) of the d
isease. Furthermore, T-614 treatment of recipient rats that had receiv
ed MBP-sensitized lymphoid cells resulted in suppression of the clinic
al severity of EAE. Immunohistological examination revealed that the n
umber of TCR alpha beta-expressing T cells and the extent of MHC class
II expression in the spinal cord of rats treated with T-614 was marke
dly reduced. In vitro study using MBP-specifIc T cells showed that the
addition of T-614 inhibited the proliferative responses of T cells an
d the production of pro-inflammatory cytokines such as IFN-gamma, IL-6
and TNF produced by T and accessory cells. Taken together, these find
ings imply that T-614 suppresses the development of EAE by inhibiting
the proliferation of autoreactive T cells and pro-inflammatory cytokin
e production not only by T cells but also by macrophages/microglia. Th
is may be attributable to the result that T-614 is more effective at t
he effector phase rather than the induction phase. Thus, this drug has
a potential value for the treatment of various T cell-mediated autoim
mune diseases including multiple sclerosis (MS) as well as rheumatoid
arthritis. (C) 1998 Elsevier Science B.V. All rights reserved.