STUDY ON OSCILLATORY BEHAVIOR AND DYNAMIC RELEASE OF ALKALOIDS OF CULTURED CATHARANTHUS-ROSEUS CELLS

During the growth of Catharanthus roseus cells, the extracellular pH, along with the both intracellular and extracellular alkaloid contents, oscillate under both long-term and short-term sampling. This study in vestigates the possibility of manipulating the extracellular pH to ind uce the release of intracellularly retained alkaloids. The results sho w that shifting the pH leads to a dynamic release of intracellular alk aloids of C roseus cells, and meanwhile has no harmful effects on alka loid synthesis of C. roseus cells. Lowering the pH can thus be an mean s to strengthen the release of intracellular alkaloids in large quanti ties.