INTEGRALITY OF BENIGN PROSTATIC HYPERPLASIA TISSUES AFTER TRANSURETHRAL THERMOTHERAPY EVIDENCED BY TRANSMITTANCE FOURIER-TRANSFORM INFRARED-SPECTROSCOPY COMBINED WITH THERMAL ANALYZER

Transmittance Fourier transform infrared microspectroscopy combined wi th differential scanning calorimetry was used to simulate the clinical ly transurethral thermotherapy of benign prostatic hyperplasia (BPH) b y in vitro isothermal determination, The simulative study to the therm otherapy of epithelium or stroma in BPH tissue was performed at 47 deg rees C for 3 h, Like clinical therapy. Whether thermal treatment can i nduce changes in the secondary structure of epithelium or stroma in BP H tissue was investigated. The results indicate that the epithelium an d stroma in BPH tissue had different protein secondary structures, due to the different compositions of the epithelium and stroma. No signif icant change was evidenced in secondary structure for each sample eith er before or after isothermal study, suggesting the integrality and sa fety of BPH thermotherapy in a 47 degrees C for 3 h treatment course.