IMMOBILIZATION OF LIPASE USING EGG-SHELL AND ALGINATE AS CARRIERS - OPTIMIZATION OF REACTION CONDITIONS

The use of egg shell and alginate as carriers for lipase were studied and the kinetic parameters K-m and V-max were found out. These values were compared with those obtained for free lipase. The reaction condit ions were optimized at a pH of 6.0-6.5, temperature at 40 degrees C an d the time of reaction for 40 minutes. 600 mu l of free enzyme, 30 num bers of alginate beads with lipase and 2.5 mg of egg shell, loaded wit h lipase. The critical reaction velocity in case of free lipase, lipas e adsorbed on egg shell and entrapped in alginate was found to be 53.7 79 I.U., 37.450 I.U., 31.543 I.U. respectively. It was found that the egg shell loaded with lipase could be reused for 8 times while the alg inate beads only for 4 times. It was finally concluded that egg shell was an efficient carrier as far as lipase was concerned, especially in presence of an insoluble substrate.