STAPHYLOCOCCUS-HOMINIS SUBSP NOVOBIOSEPTICUS SUBSP NOV., A NOVEL TREHALOSE-NEGATIVE AND N-ACETYL-D-GLUCOSAMINE-NEGATIVE, NOVOBIOCIN-RESISTANT AND MULTIPLE-ANTIBIOTIC-RESISTANT SUBSPECIES ISOLATED FROM HUMAN BLOOD CULTURES
We. Kloos et al., STAPHYLOCOCCUS-HOMINIS SUBSP NOVOBIOSEPTICUS SUBSP NOV., A NOVEL TREHALOSE-NEGATIVE AND N-ACETYL-D-GLUCOSAMINE-NEGATIVE, NOVOBIOCIN-RESISTANT AND MULTIPLE-ANTIBIOTIC-RESISTANT SUBSPECIES ISOLATED FROM HUMAN BLOOD CULTURES, International journal of systematic bacteriology, 48, 1998, pp. 799-812
A new subspecies, Staphylococcus hominis subsp. novobiosepticus, isola
ted from human blood cultures, a wound, a breast abscess and a cathete
r tip, is described on the basis of a study of 26 strains isolated bet
ween 1989 and 1996. DNA-DNA reassociation reactions, conducted under s
tringent conditions, and macrorestriction pattern analysis demonstrate
d that these strains are closely related to previously characterized S
. hominis strains belated from human skin and clinical specimens, but
are significantly divergent, S. hominis subsp. novobiosepticus can be
distinguished from S. hominis (now named S. hominis subsp. hominis) by
its combined characteristics of novobiocin resistance and failure to
produce acid aerobically from D-trehalose and N-acetyl-D-glucosamine.
Furthermore, all 26 strains of the new subspecies are resistant to nal
idixic acid, penicillin G, oxacillin, kanamycin and streptomycin, and
were either resistant or had intermediate resistance to methicillin an
d gentamicin. Most strains were also resistant to erythromycin, clinda
mycin, chloramphenicol, trimethoprim/sulfamethoxarole and ciprofloxaci
n. Based on a comparison of the sequences of a 1001 bp mecA amplificat
ion product from reference methicillin-resistant staphylococci, the me
cA gene present in S. hominis subsp. novobiosepticus was identified as
homologue A, commonly found in S. aureus and many coagulase-negative
staphylococcal species. The type strain of S. hominis subsp. novobiose
pticus is ATCC 700236(T). Descriptions of S. hominis subsp. novobiosep
ticus subsp. nov, and S. hominis subsp. hominis are given and the desc
ription of S. hominis is emended.