ANDROGEN CONCENTRATIONS AND THEIR RECEPTORS IN THE PERIURETHRAL REGION ARE HIGHER THAN THOSE OF THE SUBCAPSULAR ZONE IN BENIGN PROSTATIC HYPERPLASIA (BPH)

Benign prostatic hyperplasia (BPH) is an androgen-dependent disease th at initially develops in the inner prostate, where the highest concent rations of testosterone (T) and dihydrotestosterone (DHT) are found. I n this study, we have evaluated the cytosolic androgen receptors (ARc) , the nuclear androgen receptors (ARn), and the concentrations of T, D HT, and 3 alpha-androstanediol (3 alpha Diol) in BPH tissue to verify the existence of a possible correlation between androgens and their re ceptor concentrations. Prostatic samples, removed by suprapubic prosta tectomy in 15 untreated patients, were sectioned in periurethral, inte rmediate, and subcapsular zones. Testosterone, DHT, and 3 alpha Diol w ere evaluated by radioimmunoassay after extraction and purification on celite microcolumns, and ARc and ARn were evaluated by means of dextr an-coated charcoal method. In total tissue, mean levels of DHT, T, and 3 alpha Diol were 2,531 +/- 308, 260 +/- 36, and 403 +/- 35 pg/mg of DNA (mean a SE), respectively. Cytosolic androgen receptors, detectabl e in all cases, were 16 +/- 2.8 fmol/mg of protein (mean +/- SE), and ARn, detectable in 12 cases, were 108 +/- 15 fmol/mg of DNA (mean +/- SE). A linear correlation between DHT and 3 alpha Diol, T and DHT, and 3 alpha Diol and ARn was found. If the different regions are consider ed, the periurethral zone, site of the primitive BPH nodule, presents the highest levels of androgens and ARn with respect to the other regi ons. This relative hyperandrogenism may be responsible for the growth- promoting processes of this area, leading to urinary obstruction.