PROTON-COUPLED OLIGOPEPTIDE TRANSPORT BY RAT RENAL CORTICAL BRUSH-BORDER MEMBRANE-VESICLES - A FUNCTIONAL-ANALYSIS USING ACE-INHIBITORS TO DETERMINE THE ISOFORM OF THE TRANSPORTER

Authors
TEMPLE CS BOYD CAR
Citation
Cs. Temple et Car. Boyd, PROTON-COUPLED OLIGOPEPTIDE TRANSPORT BY RAT RENAL CORTICAL BRUSH-BORDER MEMBRANE-VESICLES - A FUNCTIONAL-ANALYSIS USING ACE-INHIBITORS TO DETERMINE THE ISOFORM OF THE TRANSPORTER, Biochimica et biophysica acta. Biomembranes, 1373(1), 1998, pp. 277-281
Citations number
19
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta. Biomembranes → ACNP
ISSN journal
00052736
Volume
1373
Issue
1
Year of publication
1998
Pages
277 - 281
Database
ISI
SICI code
0005-2736(1998)1373:1<277:POTBRR>2.0.ZU;2-3
Abstract
We demonstrate that the angiotensin-converting enzyme inhibitors enala pril and captopril inhibit the transport of D-Phe-L-Gln into PepT1-exp ressing Xenopus oocytes and into rat renal cortical brush border membr ane vesicles (BBMV). The kinetics of inhibition are competitive. Enala pril and captopril are not substrates for PepT2 (Boll et al., Proc. Na tl, Acad. Sci. 93 (1996) 284-289). Therefore we conclude that in rat r enal cortical BBMV this neutral dipeptide is transported via PepT1. (C ) 1998 Elsevier Science B.V. All rights reserved.