THE DETECTION OF TRICHINELLA WITH POLYMERASE-CHAIN-REACTION (PCR) PRIMERS CONSTRUCTED USING SEQUENCES OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) OR SEQUENCES OF COMPLEMENTARY-DNA ENCODING EXCRETORY-SECRETORY (E-S) GLYCOPROTEINS

Diagnostic PCR primers for Trichinella were constructed. Twelve pairs of primers were designed based on the sequences of random amplified po lymorphic DNA, and 4 pairs of primers were designed based on the repor ted sequences of complementary DNA encoding excretory-secretory glycop roteins. With these primers, 31 samples of DNA from different strains of Trichinella including 5 species (Trichinella spiralis, Trichinella britovi, Trichinella nativa, Trichinella nelsoni and Trichinella pseud ospiralis) and 3 phenotypes of uncertain taxonomic level (Trichinella T5, T6 and T8) were tested with PCR. Genus Trichinella can be identifi ed by 4 different primer pairs (SB147D, SB372A, SB153, or Ts43). Trich inella spiralis can be identified by the presence of a 673 bp amplicon in PCR with the primer pair SB147B. Trichinella nelsoni can be identi fied using primer pair SB147F or by the presence of 673 bp and ca. 380 bp amplicon in PCR with the primer pair SB147B. Trichinella pseudospi ralis can be identified by 2 primer pairs (SB147E or SB372B). Trichine lla T5 can be identified by the primer pair SB147G. Trichinella T8 can be identified by its positivity by the primer pair SB147C and its neg ativity by the primer pair SB372C. A group of Trichinella species (T. britovi, T. nativa and Trichinella T6) can be identified by the primer pair SB372C.