USE OF P-GLYCOPROTEIN GENE PROBES TO INVESTIGATE ANTHELMINTIC RESISTANCE IN HAEMONCHUS-CONTORTUS AND COMPARISON WITH ONCHOCERCA-VOLVULUS

A P-glycoprotein gene probe from the sheep parasitic nematode Haemonch us contortus was developed and used to analyse restriction fragment le ngth polymorphisms between susceptible isolates and isolates resistant to either benzimidazole; levamisole and benzimidazole; or benzimidazo le, ivermectin and closantel. No polymorphism could be correlated with any of the different resistances. A P-glycoprotein gene probe was als o isolated from the human nematode parasite Onchocerca volvulus and an Onchocerca-specific PCR was developed. (C) 1998 Australian Society fo r Parasitology. Published by Elsevier Science Ltd.