IN-SITU IDENTIFICATION OF MAJOR METABOLITES IN THE RED ALGA GRACILARIOPSIS-LEMANEIFORMIS USING HIGH-RESOLUTION MAGIC-ANGLE-SPINNING NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY

The content of low-molecular-weight compounds in the red alga Gracilar iopsis lemaneiformis [(Bory) Dawson, Acleto, et Foldvik] has been anal ysed in-situ using high-resolution magic angle spinning (HR-MAS) nucle ar magnetic resonance (NMR) spectroscopy. The major heteroside was sho wn to be floridoside, but digeneaside and isofloridoside were also det ected in the alga. Other major components were isethionic acid and the amino acids taurine and citrulline. The results from the HR-MAS NMR a nalysis were confirmed with high-resolution NMR spectroscopy, high-res olution fast atom bombardment mass spectrometry (FABMS) and GC-MS, on material isolated from the studied alga, but also on authentic samples .