A high-performance liquid chromatography (HPLC) method for folate mono
glutamates was toed to study folate vitamer stability in food. Folate
vitamers in fish and chicken fillets were determined raw and after ove
n-baking. Folate stability in liver sausage was also evaluated. Folate
vitamer stability in raw beef liver and strawberries was determined d
uring frozen storage. True vitamin retention (TVR) for tetrahydrofolat
e in rainbow trout and pollack after oven-baking was poor (9-60 %). TV
R for 5-methyltetrahydrofolate was above 65% for both fish species and
chicken meat. Folate vitamers were well retained in the processing of
liver sausage. Stability of folate vitamers during frozen storage of
beef fiver and strawberries (up to 7 months) was very good. HPLC was s
uccessfully used to study the effects of cooking and frozen storage on
folate vitamers. (C) 1998 Academic Press.