T. Marinhernandez et al., EARLY STAGES IN THE DEVELOPMENT OF SOMATIC EMBRYOGENESIS IN MAMMILLARIA-SAN-ANGELENSIS SANCHEZ-MEJORADA (CACTACEAE) A SEVERELY ENDANGERED SPECIES, Phyton, 62(1-2), 1998, pp. 181-186
Ovules of flowers in anthesis of irt vitro derived plants of Mammillar
ia san-angelensis Sanchez-Mejorada were cultured in a modified solid B
5 medium supplemented with 2,4-D (4 mg l(-1)) plus Kin (2 mg l(-1)). C
allus was induced from the integuments of the ovules. During the first
three months in culture, early stages of somatic embryogenesis were d
etected. Histological analysis revealed the unicellular as well as the
multicellular origin of the proembryonic structures. The presence of
phenols negatively affected the progress of somatic embryogenesis.