A COMPARISON OF 2 DIFFERENT SYSTEMS FOR CD34+ SELECTION OF AUTOLOGOUSOR ALLOGENEIC PBSC COLLECTIONS

This study compared CD34 selection procedures using the CellPro CEPRAT E(R) and the Baxter Isolex 300i(R) systems. Thirty-two procedures were performed, 19 CEPRATE and 13 Isolex, Median starting CD34 percentages were (CEPRATE/Isolex) 0.80% (range 0.24%-7.73%) and 0.85% (range 0.27 %-10.17%), respectively (p = 0.788), After selection, there was a high ly significant difference in purity of the product (CEPRATE/Isolex), 5 4% and 82% respectively (p < 0.0001). There was no significant differe nce in median recovery (CEPRATE/Isolex), 43% and 50%, respectively (p = 0.383). The starting CD34 percentage influenced the purity of the fi nal product, and at high and low starting percentages, the Isolex prod uced superior purity. Improved efficacy of T cell depletion was observ ed with the Isolex, a median log depletion of 3.4 compared with 2.9 fo r the CEPRATE system (p = 0.012). In conclusion, the Isolex 300i produ ced a significantly higher purity CD34+ fraction, even at starting CD3 4+ levels of <0.5%, with no significant difference in recovery when co mpared with the CEPRATE system. The associated log T cell depletion is significantly improved with the Isolex system, with possible implicat ions for use in CD34-selected allogeneic transplants.