Bx. Ye et al., A STUDY OF HEMOGLOBIN AT SILVER COLLOID BY SURFACE-ENHANCED RAMAN-SPECTROSCOPY, Gaodeng xuexiao huaxue xuebao, 19(8), 1998, pp. 1239-1241
The properties of Hemoglobin adsorbed on the silver colloid surface we
re investigated by using surface enhanced Raman spectroscopy (SERS) in
the determination of oxygen-hemoglobin, deoxygen-hemoglobin and heme.
The results show that the heme chromophore of oxygen-hemoglobin inter
acts directly with silver colloid, as a result of either its detachmen
t from the protein matrix or denaturation from the protein. The deoxyg
en-hemoglobin would not release its heme at the silver colloid as norm
ally expected.