A FOLATE BINDING-PROTEIN IN ASCITIC FLUID, SERUM AND OVARIAN TISSUE OF PATIENTS WITH OVARIAN ADENOCARCINOMA IMMUNOREACTS WITH ANTIBODIES AGAINST HUMAN-MILK FOLATE BINDING-PROTEIN
J. Holm et al., A FOLATE BINDING-PROTEIN IN ASCITIC FLUID, SERUM AND OVARIAN TISSUE OF PATIENTS WITH OVARIAN ADENOCARCINOMA IMMUNOREACTS WITH ANTIBODIES AGAINST HUMAN-MILK FOLATE BINDING-PROTEIN, Bioscience reports, 18(2), 1998, pp. 49-57
The presence of a folate binding protein which immunoreacts with antib
odies against human milk folate binding protein was demonstrated in as
citic fluids from seven patients with ovarian adenocarcinoma. Ascitic
fluids collected from two patients with other malignancies contained n
on-immunoreactive FBP. Tumor tissue specimens from five patients with
ovarian carcinoma contained immunoreactive FBP. By contrast to normal
ovaries ovarian carcinoma tissue showed positive immunostaining on imm
unohistochemistry. Ascitic fluids from two patients with ovarian carci
noma exhibited single distinct bands on SDS-PAGE immunoblotting. The g
el filtration profile of ovarian carcinoma tissue homogenate from two
patients contained 25 and 100 kDa peaks of radioligand-bound and immun
oreactive folate binding protein, while ascitic fluid from one of the
patients exhibited a large 100 kDa immunoreactive peak with no radioli
gand binding activity. The immunoreactive non-functional 100 kDa FBP c
ould represent unprocessed precursor FBP. Future studies are necessary
to evaluate whether determination of immunoreactive FBP in ovarian ad
enocarcinomatosis is of any diagnostic value.