ESCHERICHIA-COLI O157 SEROLOGY - FALSE-POSITIVE ELISA RESULTS CAUSED BY HUMAN-ANTIBODIES BINDING TO BOVINE SERUM-ALBUMIN

Authors
CHART H EVANS J CHALMERS RM SALMON RL
Citation
H. Chart et al., ESCHERICHIA-COLI O157 SEROLOGY - FALSE-POSITIVE ELISA RESULTS CAUSED BY HUMAN-ANTIBODIES BINDING TO BOVINE SERUM-ALBUMIN, Letters in applied microbiology, 27(2), 1998, pp. 76-78
Citations number
27
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
27
Issue
2
Year of publication
1998
Pages
76 - 78
Database
ISI
SICI code
0266-8254(1998)27:2<76:EOS-FE>2.0.ZU;2-V
Abstract
An analysis of farm workers and rural dwellers for serum antibodies to the lipopolysaccharide (LPS) of Escherichia coli O157 detected sera w ith antibodies binding to bovine serum albumin (BSA) by ELISA. These a ntibodies were not specific for BSA when examined by immunoblotting, a nd the ELISA values were reduced to a background level when plates wer e blocked with normal rabbit serum.