Bordetella bronchiseptica isolates utilised tricarboxylic acid cycle i
ntermediates succinate, citrate, alpha-ketoglutarate, fumarate, lactat
e and oxalo-acetate; the organic acids pyruvate, acetate and lactate;
and the amino acids proline, glutamate, glutamine and tyrosine - as so
le sources of carbon and energy, The inability of B, bronchiseptica is
olates, representing the three phase types and from different animal h
osts, to utilise carbohydrates and sugar alcohols as sole carbon and e
nergy sources was confirmed and extended. The influence of the carbon
substrate on doubling time, piliation, flagellation, motility capsule
production and adherence to mammalian cells was also measured.