ARTIFICIAL ENZYMATIC MEMBRANE PUMP FOR GLUCOSE-TRANSPORT AGAINST ITS CHEMICAL GRADIENT

An artificial glucokinase/phosphatase porous membrane separating two u nequal compartments of a diffusion cell was used to pump glucose from one compartment against the concentration gradient of the opposite one . Our results mainly demonstrate that, a kinase/phosphatase reactional sequence acting on both parts of a pore structure and necessarily in unstirred layers may pump a neutral solute from one compartment agains t the concentration gradient of the opposite one without any detectabl e pollution of the charged intermediary product. The corresponding the oretical analysis, which underlines the key role played by the diffusi on layers located on both parts of this bienzymic membrane and the mem brane's charge effect, was found in good agreement with the experiment al data. This study corroborates well the new kinetic model recently p roposed for primary scalar active transport of small hydrophilic molec ules involving ATP as the energy supply. (C) 1998 Elsevier Science B.V . All rights reserved.