STUDIES ON THE SUITABILITY OF RAPD AND ISTR FOR IDENTIFICATION OF PALM SPECIES (ARECACEAE)

Citation
I. Anzizar et al., STUDIES ON THE SUITABILITY OF RAPD AND ISTR FOR IDENTIFICATION OF PALM SPECIES (ARECACEAE), Taxon, 47(3), 1998, pp. 635-645
Citations number
22
Categorie Soggetti
Plant Sciences
Journal title
TaxonACNP
ISSN journal
00400262
Volume
47
Issue
3
Year of publication
1998
Pages
635 - 645
Database
ISI
SICI code
0040-0262(1998)47:3<635:SOTSOR>2.0.ZU;2-P
Abstract
66 genotypes of species in 50 palm genera were analysed by RAPD (rando m amplified polymorphic DNA) and ISTR (inverse-sequence tagged repeat analysis). Significant numbers of polymorphic DNA fragments were gener ated by both techniques, which allowed identification of all individua ls. Genetic similarities between the genotypes were established by clu ster analysis based on Jaccard's similarity coefficients. Individuals of the same genus always formed clusters, and most of the related spec ies formed subclusters. The systematics of individuals as established by morphological, cytological and ecological considerations, however, could only be partially confirmed by the molecular studies. For the pa lm species considered in this study, RAPD and ISTR represent a powerfu l tool for genotype identification and differentiation, but they may n ot be useful for analysis of genetic relationships above the genus lev el.