SYNTHESIS OF A NEW FLUOROGENIC SUBSTRATE FOR THE ASSAY OF PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE-C

Authors
RUKAVISHNIKOV AV SMITH MP BIRRELL GB KEANA JFW GRIFFITH OH
Citation
Av. Rukavishnikov et al., SYNTHESIS OF A NEW FLUOROGENIC SUBSTRATE FOR THE ASSAY OF PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE-C, Tetrahedron letters, 39(37), 1998, pp. 6637-6640
Citations number
20
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Tetrahedron letters → ACNP
ISSN journal
00404039
Volume
39
Issue
37
Year of publication
1998
Pages
6637 - 6640
Database
ISI
SICI code
0040-4039(1998)39:37<6637:SOANFS>2.0.ZU;2-F
Abstract
The synthesis of a fluorescein-derived fluorogenic substrate, D,L-myo- inositol 1-([6'- hexyloxy-3-oxo]spiro[isobenzofuran-1 (3H),9'-[9H]xant hen]-3'-yl hydrogen phosphate) sodium salt 9, for assay of activity of phosphatidylinositide-specific phospholipase C (PI-PLC), is described . This substrate is shown to be a sensitive substrate for bacterial PI -PLC in a continuous, non-radioactive assay. (C) 1998 Elsevier Science Ltd. All rights reserved.