MONOCLONAL-ANTIBODIES NEUTRALIZING THE HEMOLYTIC-ACTIVITY OF BOX JELLYFISH (CHIRONEX-FLECKERI) TENTACLE EXTRACTS

1. Three monoclonal antibodies have been produced which neutralize in vitro the haemolytic activity present in tentacle extracts of the box jellyfish (Chironex fleckeri). 2. Two of these monoclonal antibodies b ound specifically to a component of relative molecular mass 50,000 in tentacle extract on Western blots. 3. This binding only occurred when the extracts were electrophoresed under non-reducing conditions. 4. Th e third monoclonal antibody did not display binding to Western blots o f tentacle extract under any of our experimental conditions.