COLLAGENACEOUS, THIOL-CONTAINING PROTEINS OF CNIDARIAN NEMATOCYSTS - A COMPARISON OF THE CHEMISTRY AND PROTEIN DISTRIBUTION PATTERNS IN 2 TYPES OF CNIDAE
Dd. Brand et al., COLLAGENACEOUS, THIOL-CONTAINING PROTEINS OF CNIDARIAN NEMATOCYSTS - A COMPARISON OF THE CHEMISTRY AND PROTEIN DISTRIBUTION PATTERNS IN 2 TYPES OF CNIDAE, Comparative biochemistry and physiology. B. Comparative biochemistry, 106(1), 1993, pp. 115-124
1. Nematocyst structural proteins (NSP) from the sea anemones Aiptasia
pallida and Metridium senile and the siphonophore Physalia physalis a
re primarily low molecular weight collagens linked by disulfide bonds.
2. NSP patterns resolved by SDS-PAGE revealed a common, major collage
n species (40 kDa) in each nematocyst type, together with other collag
ens and non-thiol-containing proteins. 3. For each cnidarian, NSP glyc
osylation profiles were significantly different. 4. Monoclonal antibod
ies against Aiptasia NSP demonstrated a differential distribution betw
een capsule wall and thread. 5. NSP differences would account for the
diversity of morphologic and functional types.