Gn. Ranadive et al., A NEW METHOD OF TC-99M LABELING OF MONOCLONAL-ANTIBODIES THROUGH SUGAR RESIDUES - A STUDY WITH TAG-72 SPECIFIC CC-49 ANTIBODY, Nuclear medicine and biology, 20(6), 1993, pp. 719-726
We have developed a very efficient labeling technique for monoclonal a
ntibodies with technetium-99m. Oxidation of sugar residues on the IgG
class of antibodies leads to the generation of aldehyde groups which a
re further reacted with two newly developed hydrazide compounds. This
methodology introduces sulfhydryl groups on the antibody through sugar
residues which can be labeled with technetium-99m. We have studied th
e TAG-72 specific second generation antibody CC-49. The specific activ
ity of the labeled antibody was high without loss of its immunoreactiv
ity.