DETECTION OF MSPI RFLP IN HUMAN THY1 GENE BY THE POLYMERASE CHAIN-REACTION

Authors
FU JA IKEGAMI H YAMATO E KAWAGUCHI Y TAKEKAWA K FUJISAWA T NAKAGAWA Y HAMADA Y UEDA H SHEN GQ OGIHARA T
Citation
Ja. Fu et al., DETECTION OF MSPI RFLP IN HUMAN THY1 GENE BY THE POLYMERASE CHAIN-REACTION, JPN J HUM G, 40(3), 1995, pp. 279-280
Citations number
3
Categorie Soggetti
Genetics & Heredity
Journal title
Japanese journal of human genetics → ACNP
ISSN journal
09168478
Volume
40
Issue
3
Year of publication
1995
Pages
279 - 280
Database
ISI
SICI code
0916-8478(1995)40:3<279:DOMRIH>2.0.ZU;2-D
Abstract
THY1 gene encodes a cell surface glycoprotein predominantly expressed in brain and peripheral nerves. Human THY1 gene region on chromosome 1 1q23 has been implicated in susceptibility to type 1 diabetes (Wong et al., 1991). Two primers derived from the sequences flanking the polym orphic MspI site in intron 2 of the human THY1 gene (Gatti ct al., 198 8) were selected for RCP to amplify a 566 bp fragment that spans the M spI polymorphism. Polymorphism was detected by MspI digestion of the P CR product.