Sm. Jorgensen et al., 3-DIMENSIONAL IMAGING OF VASCULATURE AND PARENCHYMA IN INTACT RODENT ORGANS WITH X-RAY MICRO-CT, American journal of physiology. Heart and circulatory physiology, 44(3), 1998, pp. 1103-1114
A microcomputed tomography (micro-CT) scanner, which generates three-d
imensional (3-D) images consisting of up to a billion cubic voxels, ea
ch 5-25 mu m on a side, and which has isotropic spatial resolution, is
described. Its main components are a spectroscopic X-ray source that
produces selectable primary emission peaks at similar to 9, 18, or 25
keV and a fluorescing thin crystal plate that is imaged (at selectable
magnification) with a lens onto a 2.5 x 2.5-cm, 1,024 x 1,024-pixel,
charge-coupled device (CCD) detector array. The specimen is positioned
close to the crystal and is rotated in 721 equiangular steps around 3
60 degrees between each X-ray exposure and its CCD recording. Tomograp
hic reconstruction algorithms, applied to these recorded images, are u
sed to generate 3-D images of the specimen. The system is used to scan
isolated, intact, fixed rodent organs (e.g., heart or kidney) with th
e image contrast of vessel lumens enhanced with contrast medium. 3-D i
mage display and analysis are used to address physiological questions
about the internal structure-to-function relationships of the organs.