Jn. Alexander et al., DEVELOPMENT OF A NANO-ELECTROSPRAY MASS-SPECTROMETRY SOURCE FOR NANOSCALE LIQUID-CHROMATOGRAPHY AND SHEATHLESS CAPILLARY-ELECTROPHORESIS, Rapid communications in mass spectrometry, 12(17), 1998, pp. 1187-1191
A Fisons Quattro I electrospray ionization (ESI) source has been modif
ied to produce stable electrospray ion currents at flow rates as low a
s 50 nL/min, The original counter electrode and skimmer cone lens of t
he Fisons ESI source have been replaced with a spherical cone lens. Th
is improved source provides a greater range of x,y,z positioning of a
stainless steel tip resulting in a stable ion signal for flow rates of
50 nL/min to 2 mu L/min, A tapered stainless steel electrospray tip (
50 mu m i,d,) was evaluated for mass spectrometry using nano-liquid ch
romatography (50 pm i,d,, flow rate = 120 nL/min) and sheathless capil
lary electrophoresis, The analysis of a nonionic surfactant, octylphen
ol ethoxylate, was accomplished with both nanoscale separation techniq
ues. (C) 1998 John Wiley & Sons, Ltd.