Jh. Cardellina et al., A CHEMICAL-SCREENING STRATEGY FOR THE DEREPLICATION AND PRIORITIZATION OF HIV-INHIBITORY AQUEOUS NATURAL-PRODUCTS EXTRACTS, Journal of natural products, 56(7), 1993, pp. 1123-1129
A relatively high percentage (ca. 15 %) of aqueous extracts from terre
strial plants, cyanobacteria, and marine invertebrates and algae has e
xhibited activity in the National Cancer Institute's primary AIDS-anti
viral screen. By removal of anionic polysaccharides in a first stage o
f dereplication, we have eliminated from further consideration a consi
derable number of these extracts. However, a still substantial proport
ion of the active extracts remained, from which we wished to select an
d prioritize a small percentage for our detailed bioassay-directed fra
ctionation studies. Therefore, a chemical screening protocol, utilizin
g various solid-phase extraction cartridges, has been developed for a
second-stage dereplication and to assist in prioritization of these ex
tracts for our further investigations.