REVERSE TRANSCRIPTASE-POLYMERASE CHAIN-REACTION STUDY OF ANION EXCHANGER-2 IN CANINE TISSUES AND DIFFERENT MADIN-DARBY CANINE KIDNEY-CELL TYPES

Authors
PRIME G EBNER S MARINGREZ M
Citation
G. Prime et al., REVERSE TRANSCRIPTASE-POLYMERASE CHAIN-REACTION STUDY OF ANION EXCHANGER-2 IN CANINE TISSUES AND DIFFERENT MADIN-DARBY CANINE KIDNEY-CELL TYPES, Kidney international, 54, 1998, pp. 171-173
Citations number
9
Categorie Soggetti
Urology & Nephrology
Journal title
Kidney international → ACNP
ISSN journal
00852538
Volume
54
Year of publication
1998
Supplement
67
Pages
171 - 173
Database
ISI
SICI code
0085-2538(1998)54:<171:RTCSOA>2.0.ZU;2-B
Abstract
Reverse transcriptase-polymerase chain reaction (RT-PCR) of mRNA from canine large intestine, skeletal muscle, pancreas, kidney, and spleen and from cultured wild-type and C7 and C11 Madin-Darby canine kidney ( MDCK) cells revealed considerable variation in anion exchanger (AE)1 a nd AE2 mRNA levels between the tissues. Similar high levels of AE2 mRN A were detected in all the MDCK cell populations. AE2 in MDCK cells is probably the basolateral Cl-/HCO3- exchanger common to the principal and beta-intercalated cells.