PROTECTION BY L-2-OXOTHIAZOLIDINE-4-CARBOXYLIC ACID OF HYDROGEN PEROXIDE-INDUCED CD3-ZETA AND CD16-ZETA CHAIN DOWN-REGULATION IN HUMAN PERIPHERAL-BLOOD LYMPHOCYTES AND LYMPHOKINE-ACTIVATED KILLER-CELLS

Citation
Mm. Corsi et al., PROTECTION BY L-2-OXOTHIAZOLIDINE-4-CARBOXYLIC ACID OF HYDROGEN PEROXIDE-INDUCED CD3-ZETA AND CD16-ZETA CHAIN DOWN-REGULATION IN HUMAN PERIPHERAL-BLOOD LYMPHOCYTES AND LYMPHOKINE-ACTIVATED KILLER-CELLS, Biochemical pharmacology, 56(5), 1998, pp. 657-662
Citations number
21
Categorie Soggetti
Pharmacology & Pharmacy",Biology
Journal title
ISSN journal
00062952
Volume
56
Issue
5
Year of publication
1998
Pages
657 - 662
Database
ISI
SICI code
0006-2952(1998)56:5<657:PBLAOH>2.0.ZU;2-C
Abstract
We investigated whether L-2-oxothiazolidine-4-carboxylic acid (OTC) [i n the form of Procysteine(R), kindly donated by Transcend Therapeutics ] could protect peripheral blood lymphocytes (PBL) and lymphokine-acti vated killer (LAK) cells from CD3 zeta and CD16 zeta chain down-regula tion induced by H2O2 produced by lipopolysaccharide (LPS)-activated au tologous monocytes. OTC is known to enhance glutathione production in cells in which glutathione was depleted by reactive oxygen species. Ou r data showed that OTC induced a significant increase in CD3 zeta and CD16 zeta chain expression in peripheral blood lymphocytes and LAK cel ls, respectively, pretreated for 12 hr at 37 degrees. Moreover, OTC si gnificantly protected peripheral blood lymphocytes and LAK against dec reased zeta chain expression induced by lipopolysaccharide-activated m onocytes or the addition of H2O2 to the culture medium. Our experiment s thus suggested that alterations in signal-transducing molecules, suc h as decreased CD3 zeta and CD16 zeta expression observed in cytotoxic T lymphocytes and LAK cells in response to oxidative stress, could be prevented by the use of OTC. (C) 1998 Elsevier Science Inc.