EPITOPE MAPPING OF HUMAN PROSTATE-SPECIFIC ANTIGEN AND GLANDULAR KALLIKREIN EXPRESSED IN INSECT CELLS

Citation
K. Rajakoski et al., EPITOPE MAPPING OF HUMAN PROSTATE-SPECIFIC ANTIGEN AND GLANDULAR KALLIKREIN EXPRESSED IN INSECT CELLS, PROSTATE CANCER AND PROSTATIC DISEASES, 1(1), 1997, pp. 16-20
Citations number
29
Categorie Soggetti
Oncology,"Urology & Nephrology
ISSN journal
13657852
Volume
1
Issue
1
Year of publication
1997
Pages
16 - 20
Database
ISI
SICI code
1365-7852(1997)1:1<16:EMOHPA>2.0.ZU;2-Y
Abstract
Two human prostate gland proteases were expressed in insect cells usin g recombinant baculovirus expression system, prostate specific antigen (PSA) is an established serum marker of prostate cancer whereas the c linical utility of its close homologue, human glandular kallikrein (hK 2) is presently unknown. The production levels using Trichoplusia ni c ells were roughly 300 mu g/l and 6 mg/l for hK2 and PSA, respectively. On western-blot we estimated the size for both proteins to be approxi mately 33 kDa which was consistent with PSA purified from seminal plas ma. Nine anti-PSA monoclonal antibodies (Mabs) out of 26 tested, repre senting five independent epitopes, also reacted with hK2. The results obtained in this study may help in designing more accurate diagnostic assays for detection and monitoring of prostate cancer.