CENTRIFUGAL COUNTERCURRENT PARTITION CHROMATOGRAPHY WITH A CHAIN WINDING COLUMN

A new kind of centrifugal countercurrent partition chromatographic col umn has been developed. The shape of the column looks like the chain o f a bicycle. Using the column, retention ratio of the stationary phase was measured with three kinds of solvent systems under various experi mental conditions. The chloroform solvent systems showed retention fro m 52 to 66% when the upper phase was used as the stationary phase. The performance of the column has been successfully demonstrated in the s eparation of dinitrophenyl (DNP) amino acids, Weimeisu and an extract of branch-leaves of Taxus cuspidate.