P. Santamaria et al., FERTILIZATION STRATEGIES FOR LOWERING NITRATE CONTENT IN LEAFY VEGETABLES - CHICORY AND ROCKET SALAD CASES, Journal of plant nutrition, 21(9), 1998, pp. 1791-1803
Studies have been carried out to evaluate whether chicory (Cichorium i
ntybus L.) and rocket salad [Eruca vesicaria (L.) Cav. subsp. sativa (
Mill)] nitrate (NO3) content can be reduced by decreasing nitrate-nitr
ogen (NO3-N) in the nutrient solution or by partially replacing it wit
h ammonium-nitrogen (NH4-N) (chicory only) few days before harvest. So
illess culture method was used applying two levels of NO3, for chicory
(2 and 4 mM NO3) and only one for rocket (2 mM NO3). Five (rocket) an
d six (chicory) days before harvesting, half of the plants were grown
always in the same solutions, while the others were grown in a solutio
n with 0.25 mM N(rocket and chicory transferred from om 2 mM N) or in
a solution where 3/4 of NO3-N were replaced with NH4-N (chicory transf
erred from 4 mM N). Two cultivars of chicory were used. 'Clio' hybrid
had larger leaf area, but lower dry mass and NO3 concentration than 'F
rastagliata' chicory. For rocket and chicory alike, the change of nutr
ient solution five or six days before harvesting did not affect relati
ve growth rate and water-use efficiency, even if plants transferred in
the solution with 0.25 mM NO3 absorbed all the available N, whereas p
lants kept in the initial solution with 2 mM NO3 only took 62% (chicor
y) or 32% of N (rocket). Chicory plants transferred from 4 mM NO3-N to
1 mM NO3N plus 3 mM NH4-N absorbed 60% of available N, thanks to the
uptake of 15.7 mg NH4-N per plant day, while those grown always with 4
mM NO3 absorbed 52% of the available N. Nitrate content, expressed on
fresh matter (f.m.) basis, resulted to be remarkably high in both spe
cies when nutrient solution was kept unchanged, but it was more than h
alved when available NO3 was cut by 3/4 in the nutrient solution five
or six days before harvesting. In chicory, it decreased by 36% when NO
3-N:NH4-N ratio passed from 4:0 to 1:3.