J. Sevcik et al., ONE-RUN CHIRAL SEPARATION OF METHAMPHETAMINE AND ITS RELATED METABOLITES BY CAPILLARY-ELECTROPHORESIS, Journal of liquid chromatography & related technologies, 21(16), 1998, pp. 2473-2484
Citations number
20
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Capillary zone electrophoretic method for the one run enantioseparatio
n of methamphetamine and its related metabolites (amphetamine, 4-hydro
xymethamphetamine, 4-hydroxyamphet-amine, norephedrine and 4-hydroxyno
rephedrine) was developed. Six different cyclodextrins (alpha-cyclodex
trin, beta-cyclodextrin, hydroxypropyl-beta-cyclodextrin, dimethyl-bet
a-cyclodextrin, epi-chlorhydrin-beta-cyclodextrin polymer and gamma-cy
clodextrin) were tested. The best chiral separation of the set of stud
ied compounds was reached under the following condition: Tris-phosphat
e buffer (pH 2.5, 100mmol.L-1) with 20 mmol.mL(-1) hydroxypropyl-beta-
cyclodextrin and V = 200 V.cm(-1). The applicability of the developed
method was tested by analyses of treated urine samples of healthy volu
nteers.