ONE-RUN CHIRAL SEPARATION OF METHAMPHETAMINE AND ITS RELATED METABOLITES BY CAPILLARY-ELECTROPHORESIS

Capillary zone electrophoretic method for the one run enantioseparatio n of methamphetamine and its related metabolites (amphetamine, 4-hydro xymethamphetamine, 4-hydroxyamphet-amine, norephedrine and 4-hydroxyno rephedrine) was developed. Six different cyclodextrins (alpha-cyclodex trin, beta-cyclodextrin, hydroxypropyl-beta-cyclodextrin, dimethyl-bet a-cyclodextrin, epi-chlorhydrin-beta-cyclodextrin polymer and gamma-cy clodextrin) were tested. The best chiral separation of the set of stud ied compounds was reached under the following condition: Tris-phosphat e buffer (pH 2.5, 100mmol.L-1) with 20 mmol.mL(-1) hydroxypropyl-beta- cyclodextrin and V = 200 V.cm(-1). The applicability of the developed method was tested by analyses of treated urine samples of healthy volu nteers.