THE FIRST ANALOGS OF LNA (LOCKED NUCLEIC-ACIDS) - PHOSPHOROTHIOATE-LNA AND 2'-THIO-LNA

Authors
KUMAR R SINGH SK KOSHKIN AA RAJWANSHI VK MELDGAARD M WENGEL J
Citation
R. Kumar et al., THE FIRST ANALOGS OF LNA (LOCKED NUCLEIC-ACIDS) - PHOSPHOROTHIOATE-LNA AND 2'-THIO-LNA, Bioorganic & medicinal chemistry letters, 8(16), 1998, pp. 2219-2222
Citations number
13
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
8
Issue
16
Year of publication
1998
Pages
2219 - 2222
Database
ISI
SICI code
0960-894X(1998)8:16<2219:TFAOL(>2.0.ZU;2-2
Abstract
LNA (Locked Nucleic Acids, 1, X = O, Y = O) is a novel oligonucleotide analogue capable of recognizing complementary DNA and RNA with unprec edented thermal affinities. Synthesis of the first chemically modified LNA analogues is reported. A 9-mer phosphorothioate- LNA containing t hree LNA thymine monomers (1, X = O, Y = S, Base = thymin-1-yl) and 9- mer LNAs containing one, three or five 2'-thio-LNA monomers (1, X = S, Y = O, Base = uracil-1-yl) were able to recognize both complementary DNA and RNA with thermal affinities comparable to those of parent LNA. (C) 1998 Elsevier Science Ltd. All rights reserved.