MULTIPLE CDC2 (P34 PROTEIN-KINASE) CDNA CLONES FROM SHOOT CDNA LIBRARY OF SOYBEAN

A shoot-derived cDNA library of soybean Glycine max (L.) ci. Bragg ino culated with Bradyrhizobium japonicum was constructed for the purpose of assessing expression changes in autoregulated legume plants. To eva luate the representativeness and utility of the library, cDNA clones h omologous to yeast cdc2/CDC28 gene were isolated. Based on restriction and sequence analysis of their 5' and 3' regions, two families of gen es were found, suggesting that as many as six different cdc2 homologue s exist in soybean. These genes may have functional relevance in tissu e-specific regulation of cell division. We conclude that the library h as utility for nodulation research, especially aspects dealing with sh oot-controlled components of autoregulation.