The quality of solar, stellar, and interstellar observations is now so
high that we can see physical effects that have been assumed to be in
significant. In natural isotopic mixtures essentially all atomic lines
are asymmetric because of isotopic and hyperfine components. In astro
physics the effects of isotopic components have been generally ignored
in the case of Ti, Cr, Fe and Ni. Ignoring isotopic splitting introdu
ces the following systematic errors: (1) the use of bisectors to deter
mine velocity fields is incorrect; (2) Fourier analysis of line profil
es to determine rotational, microturbulent, and macroturbulent velocit
ies is incorrect; and (3) damping constants are overestimated. When th
e stronger components become saturated, the weak components are still
on the linear part of the curve of growth. This introduces the additio
nal systematic errors; (4) wavelengths of saturated lines are shifted;
and (5) abundances determined from equivalent widths are wrong. In ad
dition the Doppler width and Voigt profile of each isotope are signifi
cantly different. It is imperative that laboratory measurements be mad
e to determine the isotopic splitting of every energy level of every s
table isotope of every atom and ion as is now routinely done for actin
ides and for diatomic molecules. Hyperfine splitting should be measure
d as well for those isotopes with nuclear spin. Russia, the United Sta
tes, and other countries should stockpile pure stable isotopes so they
will be available for these measurements. Computer programs for analy
zing spectra including the hyperfine and isotopic splitting already ex
ist. We now need the laboratory data.