B. Magi et al., CHARGE HETEROGENEITY OF MACROPHAGE-MIGRATION INHIBITORY FACTOR (MIF) IN HUMAN LIVER AND BREAST-TISSUE, Electrophoresis, 19(11), 1998, pp. 2010-2013
Citations number
28
Categorie Soggetti
Biochemical Research Methods","Chemistry Analytical
Macrophage migration inhibitory factor (MIF) is an ubiquitous protein
playing various immunological and hormonal roles. Theoretical electrop
horetic coordinates calculated from protein sequence in the SWISS-PROT
database (AC P14174) are 12 kDa and pI 8.24. Using two-dimensional (2
-D) immunoblotting, we have detected isoelectric forms at ca. 11.9 kDa
, with pI values of 7.8 and 6.98 in human liver tissue, breast tissue
and a cell line and in preparations of human MIF expressed in E, coli.
This evidence suggests that MIF charge heterogeneity originates from
a post-translational modification not requiring eukaryote-specific enz
ymes. We have also detected in human liver a minor immunoreactive spot
at pI 6.23, which coincides with the MIF spot in the liver map in SWI
SS-2DPAGE. The pI 6.23 isoform also conceivably derives from posttrans
lational modification, as MIF is known to be encoded in the human geno
me by a single copy gene.