BIOTRANSFORMATION AND TOXICITY OF THE LIPOXYGENASE INHIBITOR 2-HYDROXY-5-METHYLLAUROPHENONE OXIME (FLM-5011) ON HEP G2 CELLS

2-Hydroxy-5-methyllaurophenone oxime (FLM 5011) is ao inhibitor :of li poxygenase with antiinflammatory and antiallergic actions. We incubate d FLM 5011 on the human immortal cell line I-ee G2 and found a similar metabolite spectrum as in rat urine and faeces. We also measured the cytotoxicity of FLM 5011 on Hep G2 :monolayers by the amido black assa y and found that the influence of cell proliferation is partly due to apoptotic activities. Although ;the metabolic activity of Hep G2 cells is lower compared to rat hepatocyte cultures they sire a suitable tes t system for biotransformation studies. Their higher proliferation rat e allows toxicity to be characterised more exactly.