CAPACITIVE IMMUNOSENSING OF POLYCYCLIC AROMATIC HYDROCARBON AND PROTEIN CONJUGATES

A capacitive immunosensor for the detection of the interaction between benzo[a]pyrene-bovine serum albumin (BaP-BSA) and a monoclonal antibo dy (designated as MAb10c10) specific to BaP has been developed. MAb10c 10 was immobilized on a gold electrode through a self-assembled monola yer of cystamine. Linear sweep voltammetry showed that the charging cu rrent decreased with BaP-BSA binding to the antibody on the electrode, and calibration curves were constructed for BaP-BSA and Pyrene-BSA in the range of 0.01 mu M-6.00 mu M. Selectivity over structurally relat ed analytes is observed.