A PARTIAL LEAST-SQUARES MULTIVARIATE CALIBRATION METHOD FOR THE SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF SPIRONOLACTONE, CANRENONE AND HYDROCHLOROTHIAZIDE

An application of a partial least squares calibration method for the s imultaneous spectrophotometric determination of spironolactone, canren one and hydrochlorothiazide is suggested. The use of the PLS for the m ulticomponent spectrophotometric determination is demonstrated on the analysis of several synthetic mixtures. It was shown that is possible to resolve complex mixtures of analytes even when they have strongly o verlapped signals. In order to check the proposed method a statistical analysis of the results was performed. The developed method was appli ed to the 232-370 nm region of the absorption spectra for the analytes and allowed the three substances to be quantified at concentrations b etween 2.0 and 15.0 mu g.ml(-1) with good results.