REDUCED PGE(2) PRODUCTION INDUCED BY LPS AFTER HYPERTHERMIA TREATMENTBY RAT GLIAL BRAIN CULTURE

Our purpose in this research was to investigate the effect of hyperthe rmia treatment (1 h incubation at 42 degrees C) on the production of P GE(2) by rat glial cell cultures, under basal conditions following sti mulation with lipopolysaccharide (LPS) and inhibition with indomethaci n, nimesulide and dexamethasone. Stimulation of rat glial cells in cul tures with LPS caused a significant elevation of PGE2 production (7-12 -fold). This stimulation was inhibited by indomethacin, nimesulide and dexamethasone. Hyperthermia treatment reduced markedly LPS stimulated PGE2 production, but had only a mild effect on basal PGE(2) productio n. (C) 1998 Elsevier Science Ltd. All rights reserved.