Ah. Monsoroburq et al., ENVIRONMENTAL-FACTORS MODULATE THE SIZE AND THE SECRETORY ACTIVITY OFTHE NOTOCHORD - A STUDY OF THE GOLGI-APPARATUS IN AVIAN EMBRYOS, Comptes rendus de l'Academie des sciences. Serie 3, Sciences de la vie, 321(8), 1998, pp. 621-631
In this study we examined the Golgi apparatus of avian notochord trans
plants excised from 2-day-old (E2) chick embryos and grafted isochroni
cally into a chick host either in a medial-ventral position, next to t
he host notochord, or in a superficial position under the ectoderm lat
erally or dorsally to the neural tube. The operated embryos were exami
ned from E2 to E8. The diameters, the cytoplasmic vacuolization and th
e immunostained Golgi apparatus were identical between the endogenous
and ventrally grafted notochords, as well as between host-and superfic
ially transplanted notochords when observed at E2. In contrast, from E
4 to E8, the size of the notochords grafted dorsally or laterally to t
he neural tube was significantly smaller than the host, while the cyto
plasmic vacuolization and the degree of fragmentation of the Golgi app
aratus were significantly less than in the host notochords. These resu
lts show that environmental and position-specific factors influence th
e developmental program and the secretory activity of the notochordal
cells. ((C) Academie des sciences/Elsevier, Paris.).