P-32 POSTLABELING DETECTION OF DNA-BASE ADDUCTS IN MICE INDUCED BY 4-(HYDROXYMETHYL)BENZENEDIAZONIUM SALT, A CARCINOGEN IN MUSHROOM AGARICUS-BISPORUS

4-(Hydroxymethyl)benzenediazonium salt (HMBD), an ingredient in the ed ible mushroom Agaricus bisporus, is carcinogenic to mice and mutagenic to bacteria. Formation of carbon-centered radical from HMBD which ind uces DNA single strand breaks and modifies guanine and adenine moietie s of DNA into 8-[4-(hydroxymethyl) phenyl]guanine and 8-[4-(hydroxy me thyl)phenyl] adenine has been shown. In the present; study HMBD was in traperitoneally administered to mice and in vivo formation of DNA base adducts in liver DNA was investigated by P-32-postlabeling assay. A r adioactive spot caused by the modified bases was observed in liver DNA 24 h after the administration which disappeared after 48 h. The base adduct formation may play a role in carcinogenesis of HMBD in mice.