GENETIC-ANALYSIS IN FUNGI USING RESTRICTION-ENZYME-MEDIATED INTEGRATION

Restriction-enzyme-mediated integration (REMI), a method for generatin g nonhomologous integration of transforming DNA into the chromosomes o f eukaryotic cells, has been used for insertion mutagenesis and other genetic studies in diverse organisms. Insertion mutations generated by REMI have facilitated the genetic dissection of developmental pathway s in Dictyostelium discoidium and the isolation of virulence factors i n several plant pathogenic fungi. Recent work indicates that REMI occu rs by nonhomologous end joining.