During Arabidopsis root development, a radial pattern of tissues is ex
tended by the meristem, These tissues form continuous layers and recen
t data suggest that tissue continuity is instrumental for constraining
the direction of signaling in a process termed channeling. In the gro
und tissue, fate-determining signals originate from contiguous cells o
f the same layer, possibly due to specific symplastic connections, Mut
ant analysis supports the hypothesis that vascular tissue continuity m
ay facilitate and depend on the directional transport of a vascular fa
te-determining signal, possibly the phytohormone auxin.