COMPARISON OF NUCLEAR MATRIX PROTEINS BETWEEN EGFR-ANTISENSE TRANSFECTED AND UNTRANSFECTED GLIOBLASTOMA CELLS

The protein composition of the nuclear matrix is both tissue and cell type specific, and it undergoes changes with differentiation and trans formation. In the present study, nuclear matrix proteins of EGFR-antis ense transfected glioblastoma cell lines, U87 and U343, were compared with untransfected cell lines using two dimensional-gel electrophoresi s. After EGFR-antisense transfection, the protein compositions of the nuclear matrices in both cell lines were different. Several nuclear pr oteins were only found in EGFR-antisense transfected cell lines. There was no difference in NuMA expression in the transfected and untransfe cted cell lines. These results suggest that EGFR-antisense reduced tum origenicity on human glioblastoma cells by changing nuclear matrix pro tein compositions.