The bifunctional autolysin gene (atl) of Staphylococcus aureus was tra
nscribed into a 4.1-kb transcript. The transcription initiation site w
as located at an adenine residue 33-nt upstream from the putative atl
start codon, Analysis using a promoter-reporter plasmid showed that pr
omoter activity increased during the exponential growth phase, The Tn5
51 insertion site of the autolysis-deficient mutant S, aureus RUSAL2 w
as located in the putative catalytic region of the glucosaminidase dom
ain.