Sk. Kim et al., GENERATION OF MUCOSAL CYTOTOXIC T-CELLS AGAINST SOLUBLE-PROTEIN BY TISSUE-SPECIFIC ENVIRONMENTAL AND COSTIMULATORY SIGNALS, Proceedings of the National Academy of Sciences of the United Statesof America, 95(18), 1998, pp. 10814-10819
We compared peripheral and mucosal primary CD8 T cell responses to inf
lammatory and noninflammatory forms of antigen in a T cell-adoptive tr
ansfer system, Immunization with the soluble antigen, ovalbumin (ova),
administered i.p. or orally without adjuvant, activated nonmucosal CD
8 T cells but did not induce cytotoxic activity. However, after activa
tion, the transferred cells entered the intestinal mucosa and became p
otent antigen-specific killers. Thus, exogenous intact soluble protein
entered the major histocompatibility complex class I antigen presenta
tion pathway and induced mucosal cytotoxic T lymphocytes. Moreover, di
stinct costimulatory requirements for activation of peripheral versus
mucosal T cells were noted in that the CD28 ligand, B7-1, was critical
for activated mucosal T cell generation but not for activation of per
ipheral CD8 T cells, The costimulator, B7-2, was required for optimum
activation of both populations, Infection with a new recombinant vesic
ular stomatitis virus encoding ovalbumin induced lytic activity in muc
osal as well as peripheral sites, demonstrating an adjuvant effect of
inflammatory mediators produced during virus infection. Generation of
antiviral cytotoxic T lymphocytes was also costimulation-dependent. Th
e results indicated that induction of peripheral tolerance via antigen
administration may not extend to mucosal sites because of distinct co
stimulatory and inflammatory signals in the mucosa.