THE EV-O-DERIVED CELL-LINE DF-1 SUPPORTS THE EFFICIENT REPLICATION OFAVIAN LEUKOSIS-SARCOMA VIRUSES AND VECTORS

The lack of a well-behaved permanent, adherent, nontransformed chicken cell line has made some experiments with avian leukosis-sarcoma virus es (ASLV) and vectors considerably more difficult. The EV-O-derived li ne, DF-I, supports the efficient replication of subgroups (A), (B), an d (C) ASLV, as well as amphotrophic murine leukemia virus and an ASLV- derived vector that has its env gene derived from the env gene from an amphotrophic murine leukemia virus. The cell line responds appropriat ely to the expression of a transforming oncogene (v-myc) to a growth s uppressor gene [p21(wail)] and can be sorted (using FAGS) ii infected by an ASLV vector that expresses GFP. (C) 1998 Academic Press.